Application

Immunoblotting (1:1000)r>Immunohistochemistry (see comments)

General description

Recognizes the ~49 kDa SAPK1 protein and the ~55 kDa SAPK2 protein phosphorylated at Thr¹⁸³ and Tyr¹⁸⁵.Antibody Target Gene Symbol: MAPK8, MAPK9 Target Synonym: AI849689, C-JUN N-TERMINAL KINASE1, JNK, JNK1, JNK1 PROTEIN KINASE, JNK1A2, JNK21B1/2, p46JNK1, p46JNK1 ALPHA, PRKM8, Sapk gamma, SAPK P46, SAPK1, SAPK1/JNK, STRESS-ACTIVATED PROTEIN KINASE-LIKE KINASE, AI851083, Jnk p54, Jnk p55, JNK-55, JNK2, JNK2A, Jnk2a2, JNK2ALPHA, JNK2B, JNK2BETA, JUNKINASE, p54a, p54aSAPK, PRKM9, SAPK, SAPK ALPHA, SAPK ALPHA 1 Entrez Gene Name: mitogen-activated protein kinase 8 Hu Entrez ID: 5599 ;-; 5601 Mu Entrez ID: 26419 ;-; 26420 Rat Entrez ID: 116554 ;-; 50658

This PhosphoDetect™ Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb is validated for use in WB, IHC for the detection of SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵).

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~49 kDa JNK1/SAPK1 protein and the ~55 kDa JNK2/SAPK2 protein phosphorylated at Thr¹⁸³ and Tyr¹⁸⁵.

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human SAPK/JNK

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Specific for the dually phosphorylated form of JNK/SAPK containing a phosphate on threonine 183 and tyrosine 185. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the proper working dilution.

Fanger, G.R., et al. 1999. Histol. Histopathol.14, 887.Gabai, V.L., et al. 1998. FEBS Lett.438, 1.

Packaging

10 tests in Plastic ampoule

Physical form

In Dulbecco′s PBS (without Mg²⁺ and Ca²⁺), 50% glycerol, 1 mg/ml BSA, pH 7.3.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)